ABSTRACT
Boesenbergia rotunda (Zingiberaceae), is a high-value culinary and ethno-medicinal plant of Southeast Asia. The rhizomes of this herb have a high flavanone and chalcone content. Here we report the genome analysis of B. rotunda together with a complete genome sequence as a hybrid assembly. B. rotunda has an estimated genome size of 2.4 Gb which is assembled as 27,491 contigs with an N50 size of 12.386 Mb. The highly heterozygous genome encodes 71,072 protein-coding genes and has a 72% repeat content, with class I TEs occupying ~67% of the assembled genome. Fluorescence in situ hybridization of the 18 chromosome pairs at the metaphase showed six sites of 45S rDNA and two sites of 5S rDNA. An SSR analysis identified 238,441 gSSRs and 4604 EST-SSRs with 49 SSR markers common among related species. Genome-wide methylation percentages ranged from 73% CpG, 36% CHG and 34% CHH in the leaf to 53% CpG, 18% CHG and 25% CHH in the embryogenic callus. Panduratin A biosynthetic unigenes were most highly expressed in the watery callus. B rotunda has a relatively large genome with a high heterozygosity and TE content. This assembly and data (PRJNA71294) comprise a source for further research on the functional genomics of B. rotunda, the evolution of the ginger plant family and the potential genetic selection or improvement of gingers.
Subject(s)
Ginger , Zingiberaceae , Biosynthetic Pathways , DNA, Ribosomal , Flavonoids , Ginger/genetics , In Situ Hybridization, Fluorescence , Microsatellite Repeats/genetics , Zingiberaceae/geneticsABSTRACT
Solid waste disposal, particularly of plastic and rubber, significantly impacts the environment and human health;thus, encouraging consumers to u sustainable alternatives is essential to ensure a resilient future. In recent decades, bio-based material research has primarily focused on bioplastics and, accordingly, current knowledge of alternative sustainable materials (such as biorubber) is fragmented, with consumer misconceptions posing a key challenge. This paper provides a comprehensive overview of the fundamentals of bio-based materials, in addition to common misconceptions about them. The findings of a public survey that aimed to assess consumers’ attitudes towards, as well as their awareness and perceptions of, conventional and sustainable alternative materials, particularly oxo-biodegradable rubbers, are also reported in this paper. Despite their unfamiliarity with the terms ‘bio-based’ and ‘oxo-biodegradable’, most respondents had a positive view of bio-based products and expressed an interest in reducing their use of conventional products in favour of sustainable alternatives. The results also revealed that consumers are willing to spend more on sustainable alternatives because they are aware of the environmental issues associated with solid waste. This study provides new insights into knowledge gaps and challenges that must be addressed to promote the prudent use of sustainable materials in a fast-changing world.
ABSTRACT
The understanding of how genetic information may be inherited through generations was established by Gregor Mendel in the 1860s when he developed the fundamental principles of inheritance. The science of genetics, however, began to flourish only during the mid-1940s when DNA was identified as the carrier of genetic information. The world has since then witnessed rapid development of genetic technologies, with the latest being genome-editing tools, which have revolutionized fields from medicine to agriculture. This review walks through the historical timeline of genetics research and deliberates how this discipline might furnish a sustainable future for humanity.